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primary renal epithelial cells  (ATCC)


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    ATCC primary renal epithelial cells
    A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney <t>epithelial</t> cells) samples and functional drug classes.
    Primary Renal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 113 article reviews
    primary renal epithelial cells - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "Site-Dependent Decoupling of Drug-Biomarker Associations in Clear Cell Renal Cell Carcinoma Revealed by Functional Profiling of Patient-Derived Cell Models"

    Article Title: Site-Dependent Decoupling of Drug-Biomarker Associations in Clear Cell Renal Cell Carcinoma Revealed by Functional Profiling of Patient-Derived Cell Models

    Journal: bioRxiv

    doi: 10.64898/2026.04.23.720088

    A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney epithelial cells) samples and functional drug classes.
    Figure Legend Snippet: A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney epithelial cells) samples and functional drug classes.

    Techniques Used: Functional Assay, Derivative Assay, Control

    Related Articles

    Recombinant:

    Article Title: Characterisation of vaginal Lactobacillus isolates from South African women towards the development of a biotherapeutic to optimise the vaginal microbiome
    Article Snippet: .. Vaginal epithelial cells (VK2/E6E7 ATCC® CRL-2616 TM ) were maintained in complete keratinocyte serum free media (KSFM) supplemented with 0.4 mM calcium chloride, 0.05 mg/ml of bovine pituitary extract, 0.1 ng/ml human recombinant epithelial growth factor and 50 IU/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich). ..

    Article Title: Bacteriophage-mediated reduction of uropathogenic E. coli from the urogenital epithelium
    Article Snippet: .. Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were grown in keratinocyte serum-free medium (KSFM) containing 50 mg/mL bovine pituitary extract and 0.1 ng/mL human recombinant epithelial growth factor. .. Human bladder epithelium carcinoma cells (5637, ATCC HTB-9) were grown in RPMI-1640 (Corning) containing 10% heat-inactivated fetal bovine serum.

    Article Title: Bacteriophage-mediated reduction of uropathogenic E. coli from the urogenital epithelium.
    Article Snippet: .. Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were grown in keratinocyte serum-free medium (KSFM) containing 50 mg/mL bovine pituitary extract and 0.1 ng/mL human recombinant epithelial growth factor. .. Human bladder epithelium carcinoma cells (5637, ATCC HTB-9) were grown in RPMI-1640 (Corning) containing 10% heat-inactivated fetal bovine serum.

    Article Title: Bacteriophage-mediated reduction of uropathogenic E. coli from the urogenital epithelium
    Article Snippet: .. Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were grown in keratinocyte serum-free medium (KSFM) containing 50 mg/mL bovine pituitary extract and 0.1 ng/mL human recombinant epithelial growth factor. .. Human bladder epithelium carcinoma cells (5637, ATCC HTB-9) were grown in RPMI-1640 (Corning) containing 10% heat-inactivated fetal bovine serum.

    other:

    Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis
    Article Snippet: The cytolytic capacity of T. vaginalis can be measured by the release of the lactate dehydrogenase (LDH) enzyme from human vaginal epithelial cells (HMVII lineage). shows that co-incubation of the co-culture of ATCC T. vaginalis isolate (ATCC30236, at initial density 1.0 x 10 6 trophozoites/mL), C. albicans (CA3, 3.33 x 10 4 CFU/mL), and L. crispatus (LC2 5.53 x 10 6 CFU/mL) with vaginal cells led to a significant increase in the LDH release compared to the co-incubation of ATC30236 alone with vaginal cells.

    Incubation:

    Article Title: Selective Human-Milk-Inspired Antimicrobial Peptides for the Treatment of Bacterial Vaginosis.
    Article Snippet: .. Using monolayers of vaginal epithelial cells, VK2/E6E7, established in DMEM/10% FBS, and cervical epithelial cells and HeLa (ATCC, CRM-CCL-2) established in EMEM/10% FBS, and an initial seeding density of 5 × 104 cells/mL, we incubated the AMPs in a dosedependent manner (1–10 mg/mL) for 36 h prior to measurement of cellular viability via Calcein dye (L3224, Invitrogen, Carlsbad, CA, USA), as per the manufacturer’s instructions, and excitation λ = 485 nm, emission λ = 528 nm). ..

    Article Title: Selective Human-Milk-Inspired Antimicrobial Peptides for the Treatment of Bacterial Vaginosis
    Article Snippet: .. Using monolayers of vaginal epithelial cells, VK2/E6E7, established in DMEM/10% FBS, and cervical epithelial cells and HeLa (ATCC, CRM-CCL-2) established in EMEM/10% FBS, and an initial seeding density of 5 × 10 4 cells/mL, we incubated the AMPs in a dose-dependent manner (1–10 mg/mL) for 36 h prior to measurement of cellular viability via Calcein dye (L3224, Invitrogen, Carlsbad, CA, USA), as per the manufacturer’s instructions, and excitation λ = 485 nm, emission λ = 528 nm). ..



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    A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney <t>epithelial</t> cells) samples and functional drug classes.
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    A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney <t>epithelial</t> cells) samples and functional drug classes.
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    Ability of Lactobacillus crispatus and Lactobacillus gasseri to enhance human β-defensin-2 ( HβD2 ) mRNA expression was associated with its binding capacity to vaginal <t>epithelial</t> cells. L. crispatus or L. gasseri was incubated with VK2/E6E7 at a multiplicity of infection (MOI) of 50 for ( A ) 4 or ( B ) 6 h. A , Nuclei of both bacterial and host cells were stained blue with DAPI. White scale bar represents 10 μm. Microscopic bacterial cell counts were performed on nine images per sample. B , A correlation is highlighted between induced HβD2 mRNA expression and the number of L. crispatus strains that adhere to vaginal epithelial cells. Dotted lines show the 95% confidence bands of the best-fit lines. All experiments were conducted in at least three independent trials. Each dot indicates the mean values. The correlation was evaluated using Spearman's correlation coefficient; **** indicates statistically significant differences ( P < .0001).
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    Image Search Results


    A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney epithelial cells) samples and functional drug classes.

    Journal: bioRxiv

    Article Title: Site-Dependent Decoupling of Drug-Biomarker Associations in Clear Cell Renal Cell Carcinoma Revealed by Functional Profiling of Patient-Derived Cell Models

    doi: 10.64898/2026.04.23.720088

    Figure Lengend Snippet: A. Drug sensitivity scores of representative PDC models for the drugs exhibiting mean DSS > 10 in at least one sample. Tumor cell fraction estimates, drug plate, passage, sample type, site and either presence or absence of commonly observed ccRCC mutations are indicated at the top of the heatmap while the functional drug class of each drug is indicated on the left. All DSRT replicates were averaged (mean) per matching WES sample (including replicates screened in 2D/3D conditions with FS2A (116 drugs)/FO5A (528 drugs) plates. For P1, P15 and P24, overall lower DSS values were observed in 3D-than 2D-DSRT). B. Intra-patient variability in drug sensitivity profiles of samples derived from T, VC, and MET for patients P1 and P10. C. Median DSS across T, MET, VC, cell line, B, and control (primary kidney epithelial cells) samples and functional drug classes.

    Article Snippet: Cortical (PCS-400-011, ATCC) and proximal (PCS-400-010, ATCC) primary renal epithelial cells, together with ACHN (CRL-1611, ATCC), A-498 (ACC 55, DSMZ), Caki-1 (ACC 731, DSMZ), Caki-2 (ACC 54, DSMZ), and 786-O (CRL-1932, ATCC) cell lines, were cultured and maintained in accordance with the manufacturers’ instructions.

    Techniques: Functional Assay, Derivative Assay, Control

    Ability of Lactobacillus crispatus and Lactobacillus gasseri to enhance human β-defensin-2 ( HβD2 ) mRNA expression was associated with its binding capacity to vaginal epithelial cells. L. crispatus or L. gasseri was incubated with VK2/E6E7 at a multiplicity of infection (MOI) of 50 for ( A ) 4 or ( B ) 6 h. A , Nuclei of both bacterial and host cells were stained blue with DAPI. White scale bar represents 10 μm. Microscopic bacterial cell counts were performed on nine images per sample. B , A correlation is highlighted between induced HβD2 mRNA expression and the number of L. crispatus strains that adhere to vaginal epithelial cells. Dotted lines show the 95% confidence bands of the best-fit lines. All experiments were conducted in at least three independent trials. Each dot indicates the mean values. The correlation was evaluated using Spearman's correlation coefficient; **** indicates statistically significant differences ( P < .0001).

    Journal: Open Forum Infectious Diseases

    Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

    doi: 10.1093/ofid/ofag193

    Figure Lengend Snippet: Ability of Lactobacillus crispatus and Lactobacillus gasseri to enhance human β-defensin-2 ( HβD2 ) mRNA expression was associated with its binding capacity to vaginal epithelial cells. L. crispatus or L. gasseri was incubated with VK2/E6E7 at a multiplicity of infection (MOI) of 50 for ( A ) 4 or ( B ) 6 h. A , Nuclei of both bacterial and host cells were stained blue with DAPI. White scale bar represents 10 μm. Microscopic bacterial cell counts were performed on nine images per sample. B , A correlation is highlighted between induced HβD2 mRNA expression and the number of L. crispatus strains that adhere to vaginal epithelial cells. Dotted lines show the 95% confidence bands of the best-fit lines. All experiments were conducted in at least three independent trials. Each dot indicates the mean values. The correlation was evaluated using Spearman's correlation coefficient; **** indicates statistically significant differences ( P < .0001).

    Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Binding Assay, Incubation, Infection, Staining

    Lactobacillus crispatus -induced human β-defensin-2 ( HβD2 ) mRNA expression in cervical epithelial cells, but not in endometrial epithelial cells. Heat-killed L. crispatus was incubated with ( A ) A2EN and ( B ) HEC-1A cells at a multiplicity of infection (MOI) of 50 for 6 h. After extracting RNA, cDNA was prepared, and quantitative PCR was performed to measure the mRNA levels for HβD2 or GAPDH . Each value was normalized to GAPDH mRNA levels. Normalized value in Dulbecco's phosphate-buffered saline (DPBS)-treated cells was set to 1. Vertical axis indicates the relative changes compared with the DPBS control of HβD2 mRNA. All experiments were conducted in at least four independent trials. Vertical bars represent mean ± standard deviations. Statistically significant difference (*; P < .05) is presented based on unpaired t -test results (A: DPBS vs HMS-115 Heat-killed; P = .0105).

    Journal: Open Forum Infectious Diseases

    Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

    doi: 10.1093/ofid/ofag193

    Figure Lengend Snippet: Lactobacillus crispatus -induced human β-defensin-2 ( HβD2 ) mRNA expression in cervical epithelial cells, but not in endometrial epithelial cells. Heat-killed L. crispatus was incubated with ( A ) A2EN and ( B ) HEC-1A cells at a multiplicity of infection (MOI) of 50 for 6 h. After extracting RNA, cDNA was prepared, and quantitative PCR was performed to measure the mRNA levels for HβD2 or GAPDH . Each value was normalized to GAPDH mRNA levels. Normalized value in Dulbecco's phosphate-buffered saline (DPBS)-treated cells was set to 1. Vertical axis indicates the relative changes compared with the DPBS control of HβD2 mRNA. All experiments were conducted in at least four independent trials. Vertical bars represent mean ± standard deviations. Statistically significant difference (*; P < .05) is presented based on unpaired t -test results (A: DPBS vs HMS-115 Heat-killed; P = .0105).

    Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Incubation, Infection, Real-time Polymerase Chain Reaction, Saline, Control

    Impact of Lactobacillus crispatus bacterial viability on vaginal epithelial cells. L. crispatus was incubated with VK2/E6E7 cells at a multiplicity of infection (MOI) of 50 for 6 h. After extracting RNA from VK2/E6E7 cells, cDNA was prepared, and quantitative PCR was performed to measure mRNA levels for IL8 ( A ), TNFα ( B ), and MUC1 ( C ). Each value was normalized to GAPDH mRNA levels, with the value in Dulbecco's phosphate-buffered saline (DPBS)-treated cells set to 1. Vertical axis represents the relative changes for each target mRNA compared with the DPBS control, while the horizontal axis shows the live or heat-killed L. crispatus HMS-115. All experiments were conducted in three independent trials. Vertical bars represent mean ± standard deviations. Statistically significant differences (**; P < .01, ***; P < .001, ****; P < .0001) are presented based on one-way analysis of variance followed by Tukey's multiple comparison test (A: DPBS vs HMS-115 Live; P = .0002, HMS-115 Live vs Heat-killed; P < .0001, B: DPBS vs HMS-115 Live; P < .0001, HMS-115 Live vs Heat-killed; P < .0001, C: DPBS vs HMS-115 Heat-killed; P = .0034, HMS-115 Live vs Heat-killed; P = .0061).

    Journal: Open Forum Infectious Diseases

    Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

    doi: 10.1093/ofid/ofag193

    Figure Lengend Snippet: Impact of Lactobacillus crispatus bacterial viability on vaginal epithelial cells. L. crispatus was incubated with VK2/E6E7 cells at a multiplicity of infection (MOI) of 50 for 6 h. After extracting RNA from VK2/E6E7 cells, cDNA was prepared, and quantitative PCR was performed to measure mRNA levels for IL8 ( A ), TNFα ( B ), and MUC1 ( C ). Each value was normalized to GAPDH mRNA levels, with the value in Dulbecco's phosphate-buffered saline (DPBS)-treated cells set to 1. Vertical axis represents the relative changes for each target mRNA compared with the DPBS control, while the horizontal axis shows the live or heat-killed L. crispatus HMS-115. All experiments were conducted in three independent trials. Vertical bars represent mean ± standard deviations. Statistically significant differences (**; P < .01, ***; P < .001, ****; P < .0001) are presented based on one-way analysis of variance followed by Tukey's multiple comparison test (A: DPBS vs HMS-115 Live; P = .0002, HMS-115 Live vs Heat-killed; P < .0001, B: DPBS vs HMS-115 Live; P < .0001, HMS-115 Live vs Heat-killed; P < .0001, C: DPBS vs HMS-115 Heat-killed; P = .0034, HMS-115 Live vs Heat-killed; P = .0061).

    Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Incubation, Infection, Real-time Polymerase Chain Reaction, Saline, Control, Comparison

    Schematic representation of the interaction between vaginal Lactobacillus crispatus and human β-defensin-2 (HβD-2) production. HβD-2 mRNA expression and production in vaginal epithelial cells varied significantly across L. crispatus strains. Vaginal L. crispatus with strong adhesion capability increased HβD-2 production in vaginal epithelial cells.

    Journal: Open Forum Infectious Diseases

    Article Title: Induction of Human β-Defensin-2 by Vaginal Lactobacillus crispatus Strains in Vaginal Epithelial Cells Correlates With Their Adhesion Abilities

    doi: 10.1093/ofid/ofag193

    Figure Lengend Snippet: Schematic representation of the interaction between vaginal Lactobacillus crispatus and human β-defensin-2 (HβD-2) production. HβD-2 mRNA expression and production in vaginal epithelial cells varied significantly across L. crispatus strains. Vaginal L. crispatus with strong adhesion capability increased HβD-2 production in vaginal epithelial cells.

    Article Snippet: VK2/E6E7 (ATCC CRL-2616), primary immortalized human vaginal epithelial cells, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing